GFkV Elisa dasi

Product components

DASI ELISA KIT grapevine fleck virus

capture polyclonal- detection monoclonal line 117 – conjugate anti mouse - positive and negative controls

Availability: In stock

Product Name Product Code Price Qty
GFkV Elisa dasi - 500 tests K-09B
GFkV Elisa dasi - 1000 tests K-09A
elisa dasi kit

Data Sheet

Method of assay ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 117, produced by DPPMA, University of Bari, Italy
Diagnostic signal colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate
Test number
Intended Use in vitro detection of grapevine fleck virus (GFkV) in grapevine phloem and young leaf
Specificity Boscia D., V. Elicio, V. Savino and G.P. Martelli, 1994. Production of monoclonal antibodies to grapevine fleck virus. Plant Pathology 44, 160-163
Supplied controls Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GFkV were ascertained by indexing on Vitis indicators, by ELISA and PCR
Supplied reagents Coating Pab 200 µl/well Revealing Mab 200 µ/well Conjugate Anti mouse – AP 200 µl/well
Shelf life 9-12 months when stored at 4°C

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