Data Sheet
| Method of assay | ELISA Double Antibody Sandwich (DAS) by monoclonal antibody line F5 produced by DIBCA, University of Bari, Italy |
|---|---|
| Diagnostic signal | colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate |
| Test number | |
| Intended Use | in vitro detection of grapevine virus A (GVA) in grapevine phloem |
| Specificity | Boscia D., E. Aslouj, V. Elicio, V. Savino, M.A. Castellano and G.P. Martelli, 1992. Production,characterization and use of monoclonal antibodies to grapevine virus A. Archives of Virology 127, 184-194 |
| Supplied controls | Positive 2 wells/plate Negative 2 wells/plate Freeze-dried grapevine phloem. The presence and the absence of GVA were ascertained by indexing on Vitis indicators, by ELISA and PCR |
| Supplied reagents | capture 100 µl/well - conjugate 100 µ/well |
| Shelf life | 9-12 months since the packaging date when stored at 4°C |
| Sensibility |
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