Xylella fastidiosa Elisa

Product components

DAS ELISA KIT Xylella fastidiosa (Wells and Raju)

developed against subspecies pauca strain CoDiRO

capture polyclonal - conjugate polyclonal - positive and negative controls

Availability: In stock

Product Name Product Code Price Qty
Xylella fastidiosa Elisa - 1000 tests K-27A
€300.00
Xylella fastidiosa Elisa - 500 tests K-27B
€170.00

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Data Sheet

Method of assay ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies
Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number 500 or 1000
Intended Use

in vitro detction of Xylella fastidiosa (Wells and Raju) in olive tree leaves 

Agritest test performances available in Appendix 1 of the OEPP/EPPO PM7/24 (2) Xylella fastidiosa

Specificity

X. fastidiosa subsp. fastidiosa, multiplex, pauca

Polyclonal antibodies raised against subsp. pauca strain CoDiRO

  • Giampetruzzi, A., Chiumenti, M., Saponari, M., Donvito, G., Italiano, A., Loconsole, G., Boscia, D., Cariddi, C., Martelli, G. P. and Saldarelli, P. 2015. Draft genome sequence of Xylella fastidiosa CoDiRO strain. Genome Announc 3(1):e01538-14. doi: 10.1128/genomeA.01538-14. http://genomea.asm.org/content/3/1/e01538-14.full.pdf+html

 

No ELISA cross reaction found against the plant pathogens:

Agrobacerium tumefaciens biovars 1, 2, A.vitis; Brenneria populi, B. quercina, B. rubrifaciens; Burkholderia andropogonis; Clavibcter michiganensis subsp. michiganensis; Erwinia amylovora, Pantoea agglomerans; Pantoea stewartii subsp stewartii; Pseudomonas amygdali, P. marginalis pv marginalis, P. savastanoi pv savastanoi , P. s. pv syringe, P. s. pv garcae; Ralstonia solanacearum; Xanthomonas arboricola pv celebensis, X. a. pv corylina, X. a. pv juglandis, X. a. pv pruni; Xanthomonas campestris pv citri, X. c. pv populi, X. c. pv vesicatoria, X. c. pv viticola, X. hortorum pv pelrgonii  Data produced by CREA-PAV, Rome, Italy.

Supplied controls 1 positive freeze-dried inactivated bacteria extract - 1 negative freeze-dried healthy olive leaf extract
Supplied reagents capture antibodies 200 ul/well - conjugate antibodies 200 ul/well
Shelf life 9-12 months when stored at 4°C
Sensibility 10^5-10^4 cfu/ml PBS
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