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Diagnostic kits are based on serological and molecular methods, taking into account: the state-of-art of plant pathology, the needs of our customers and the purposes of the analysis.

Technology assessment and transfer is conducted under a collaborative agreement between Agritest and national research centers, since 1998:

  • University of Bari Dipartimento di Scienze del Suolo della Pianta e degli Alimenti (DiSSPA),

  • CNR, Institute for Sustainable Plant Protection (IPSP),

  • Centro di Ricerca, Sperimentazione e Formazione in Agricoltura Basile Caramia (CRSFA),

  • Mediterranean Agronomic Institute of Bari (IAM-CHIEAM).

 

Antibodies

The antibodies for the ELISA reagents are raised against proteins derived from plant pathogens of recognized research center collections in Italy. The homologous antigen and its variants are used to assess the assay performances. Pathogen recombinant proteins are also exploited to surrogate the internal reference material and strictly evaluated for their consistency against pathogen-infected samples.

Molecular probes

Dot-blot DNA hybridization method has been preferred as suitable for the simultaneous detection of multiple targets. The exploited sets of technology are:

Patent pending

Synthesis and use of a polyprobe for the detection of viral infections in globe artichoke” (EPO application n° 14002761.6), Agritest srl, 2014.

 

In licensing “Diagnostic set and method to detect fitovirus infections in nursery productions”. Italian patent n° 0001355079, University of Bari, 2004.

 

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