PPV universal ELISA

300.00

plum pox virus

capture polyclonal
detection monoclonal line 5B
conjugate polyclonal anti mouse
positive and negative controls

  • K-10B
  • PPV universal ELISA KIT 500 T
  • € 300,00
  • K-10A
  • PPV universal ELISA KIT 1000 T
  • € 540,00

SKU K-10 Category

Data Sheet

TitleDescription
Method of assay

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 5B supplied by Instituto Valenciano de Investigaciones Agrarias (IVIA), Spagna

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 - 1000

Intended Use

in vitro detection of plum pox virus (PPV) in leaf of stone fruit tree

Specificity
  • Cambra M., Asensio M., Gorris M.T., Perez E., Camarasa E., Garcia J.A.,Moya J.J., Lopez-Abella D., Vela C., and Sanz A.,1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bulletin 24, 569-577
  • PPV cherry, Dideron, El Amar, Marcus, Recombinant, Winona (internal data 2011)
Supplied controls

1 Positive- 1 Negative freeze-dried peach leaf. The presence and the absence of PPV were ascertained by indexing on peeach indicator, ELISA and PCR

Supplied Reagents

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life

9-12 months when stored at 4°C

Sensitivityn.p.

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