GLRaV-1+3 ELISA

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of Grapevine leafroll associated virus 1 (GLRaV-1) and Grapevine leafroll associated virus 3 (GLRaV-3) in grapevine samples

• Boscia D., Greif C., Gugerli P., Martelli G.P., Walter B., and Gonsalves D., 1995. Nomenclature of grapevine leaf-roll associated putative closterovirus. Vitis 34:171-175.
• Gugerli,P. 1991. Grapevine closterovirus, p.40-51. In I.C. Rumbos, R. Bovey, D. Gonsalves, W.B. Hewitt, and G.P. Martelli (ed.), Proceedings of the 10th Meeting of the International Council for the Study of Viruses and Virus Diseases of the Grapevine (ICVG). Plant Protection Institute, P.O. Box 303, 38001 Volos, Greece.

1 positive GLRaV-1- 1 positive GLRaV-2- 1 negative freeze-dried. The absence and the presence of the virus were ascertained by indexing, ELISA, PCR.

capture 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C

n.p.