Company

Agritest S.r.l. is a micro-enterprise specialized in developing and manufacturing diagnostic kits for plant pathogen detection. Established in 1993, Agritest S.r.l. is privately held by the management.  Management and personnel were trained plant pathology, biotechnology, and agriculture science in academic and industrial research centers.

Products

The Agritest diagnostics are ELISA and DTBIA immunoassay kits, based on antibody reagents specific to plant pathogen proteins. With the objective of providing diagnostic validated tests, Agritest is committed to characterize, assess and maintain plant disease reference material by collaborating with scientific institutions. 

The product catalog is built up for supplying the market sectors of grapevine, citrus, fruit trees, and olive cultures giving priority to the plant diseases affecting agricultural systems in terms of economic and environmental damages. Consistently, Agritest has developed unique tests to support the competitiveness of the nursery industry, such as grapevine virus A and B, plum pox virus Marcus strain, citrus psorosis virus, citrus concave gumassociated virus. 

In order to facilitate the workflow of the testing laboratories, special attention is given to implement the EPPO guidelines to assess, along the time, the diagnostic performances of the tests. On that regard, under the activities of the EU Horizon 2020, POnTE project, Agritest was involved in carrying out proficiency tests including the evaluation of the Xylella fastidiosa ELISA kit (K-27).

Laboratory

Agritest facility is located in the technological park of the University of Bari, Tecnopolis, Valenzano, Italy.

The laboratory is fully equipped and organized to conduct the whole manufacturing cycle of the antibody-based products:

  • plant virus purification and antigen preparation
  • production of antigenic recombinant proteins
  • hybridoma culture
  • preparation of antibody reagents
  • calibration of assay controls against reference material
  • assessment of diagnostic performances
  • Post-sales quality control

Research & Development

Agritest expertise and innovation perspective develop by collaborating with Italian research institutions (University departments of plant pathology, and plant protection units of the National Council of Research-CNR). Technology transfer and knowledge exchange are implemented by means of research projects partnerships, IPR management, MTA agreements, testimonial educational lessons.

Latest references:

PONTE

Sustain management of emerging diseases and alien pests

DISCOVER

TROPICAL

Sustain management of emerging diseases and alien pests

DISCOVER

Funded projects EU Horizon 2020

MTA agreements

  • Consiglio Nazionale delle Ricerche, Institute for Sustainable Plant Protection, Bari (IPSP-BA), 2021.

Educational

  • Università degli Studi “Aldo Moro” di Bari, Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti (DISSPA), Regione Puglia, POC PUGLIA FESR-FSE 2014 / 2020, RIPARTI program, 2022.
  • Università del Salento, Dipartimento di Matematica e Fisica, Regione Puglia, POC PUGLIA FESR-FSE 2014 / 2020, RIPARTI program, 2022.

Scientific publications

  • Contaldo N., Freo I., Elicio V., Lucchese C., Formica L., Bertaccini A. 2019. Preliminary evaluation of the use of an antiserum obtained from phytoplasma culture. Phytopathogenic Mollicutes 9(1), 201-202.
  • Filippin L., Trivellone V., Galetto L., Marzachí C., Elicio V., Angelini E. 2019. Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants. Phytopathogenic Mollicutes 9(1), 75-76.
  • Loconsole, G.; Zicca, S.; Manco, L.; El Hatib, O.; Altamura, G.; Potere, O.; Elicio, V.; Valentini, F.; Boscia, D.; Saponari, M. Diagnostic Procedures to Detect Xylella fastidiosa in Nursery Stocks and Consignments of Plants for Planting. Agriculture 2021, 11, 922. https://doi.org/10.3390/agriculture11100922
  • Sarcina, L., Macchia, E., Loconsole, G., D’Attoma, G., Saldarelli, P., Elicio, V., Palazzo, G. and Torsi, L. (2021), Surface Plasmon Resonance Assay for Label-Free and Selective Detection of Xylella Fastidiosa. Adv. NanoBiomed Res., 1: 2100043. https://doi.org/10.1002/anbr.202100043
  • Buja, I.; Sabella, E.;Monteduro, A.G.; Rizzato, S.;De Bellis, L.; Elicio, V.; Formica, L.;Luvisi, A.; Maruccio, G. Detection of Ampelovirus and Nepovirus by Lab-On-A-Chip: A Promising Alternative to ELISA Test for Large Scale Health Screening of Grapevine.Biosensors 2022, 12, 147. https://doi.org/10.3390/bios12030147