Xylella fastidiosa ELISA

Direct tissue blot immunoassay (DTBIA) by polyclonal antibodies raised against inactivate bacteria extracts of X. fastidiosa subsp. pauca strain CoDiRO

Colorimetric by the reaction of alkaline phosphatase with BCIP-NBT substrate

92 sectors

in vitro detection of Xylella fastidiosa (Wells and Raju) in olive tree twigs

Agritest test performances available in Annex 1 of the OEPP/EPPO PM7/24 (3) Xylella fastidiosa

X. fastidiosa subspecies fastidiosa, multiplex, pauca

Polyclonal antibodies rased against subsp. pauca strain CoDiRO

* Giampetruzzi et al., 2015 http://genomea.asm.org/content/3/1/e01538-14.full.pdf+html 

* Djelouah K., et al. 2014. Direct tissue blot immunoassay for detection of Xylella fastidiosa in olive trees. Phytopathologi Maditerranea 53, 559-564.

1 positive spot of inactivate bacteria extract; 1 negative print of olive twigs, bacteria free

20 ml final volume

9-12 months when stored at 4°C

n°8 prints/olive sample