Xylella fastidiosa ELISA


Xylella fastidiosa (Wells and Raju)

capture polyclonal
conjugate polyclonal
positive and negative controls

  • K-27B
  • X. fastidiosa ELISA KIT 500 T
  • € 180,00
  • K-27A
  • X. fastidiosa ELISA KIT 1000 T
  • € 300,00

SKU k-27 Category

Data Sheet

Method of assay

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 or 1000

Intended Use

in vitro detction of Xylella fastidiosa (Wells and Raju) in olive tree leaves 

Agritest test performances available in Appendix 1 of the OEPP/EPPO PM7/24 (3) Xylella fastidiosa


X. fastidiosa subsp. fastidiosa, multiplex, pauca

Polyclonal antibodies raised against subsp. pauca strain CoDiRO
  • Giampetruzzi, A., Chiumenti, M., Saponari, M., Donvito, G., Italiano, A., Loconsole, G., Boscia, D., Cariddi, C., Martelli, G. P. and Saldarelli, P. 2015. Draft genome sequence of Xylella fastidiosa CoDiRO strain. Genome Announc 3(1):e01538-14. doi: 10.1128/genomeA.01538-14. http://genomea.asm.org/content/3/1/e01538-14.full.pdf+html

No ELISA cross reaction found against the plant pathogens: Agrobacerium tumefaciens biovars 1, 2, A.vitis; Brenneria populi, B. quercina, B. rubrifaciens; Burkholderia andropogonis; Clavibcter michiganensis subsp. michiganensis; Erwinia amylovora, Pantoea agglomerans; Pantoea stewartii subsp stewartii; Pseudomonas amygdali, P. marginalis pv marginalis, P. savastanoi pv savastanoi , P. s. pv syringe, P. s. pv garcae; Ralstonia solanacearum; Xanthomonas arboricola pv celebensis, X. a. pv corylina, X. a. pv juglandis, X. a. pv pruni; Xanthomonas campestris pv citri, X. c. pv populi, X. c. pv vesicatoria, X. c. pv viticola, X. hortorum pv pelrgonii  Data produced by CREA-PAV, Rome, Italy.

Supplied controls

1 positive freeze-dried inactivated bacteria extract - 1 negative freeze-dried healthy olive leaf extract – negative controls of healthy fruit trees are available upon request

Supplied Reagents

capture antibodies 200 ul/well - conjugate antibodies 200 ul/well

Shelf life

9-12 months when stored at 4°C


10^5-10^4 cfu/ml PBS

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