ArMV + GFLV ELISA

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro simultaneous detection of Arabis mosaic virus (ARMV) and Grapevine fanleaf virus (GFLV) in grapevine samples

Murant A.F.,A.T. Jones, G. P. Martelli, and R. Stace-Smith. 1996. Nepoviruses: General properties, diseases and virus identifications, p.99-137. In B.D. Harrison and A. F. Murant (ed.), Plant Viruses, Vol 5. Plenum press, London and New York.

1 positive ARMV – 1 positive GFLV - 1 negative freeze-dried. The absence and the presence of the virus were ascertained by ELISA, PCR.

capture 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C

n.p.