ApMV ELISA

300.00

apple mosaic virus

capture polyclonal
detection monoclonal line B11
conjugate polyclonal anti mouse
positive and negative controls

  • K-15B
  • APMV ELISA KIT 500 T
  • € 180,00
  • K-15A
  • APMV ELISA KIT 1000 T
  • € 330,00

SKU K-15A Category

Data Sheet

TitleDescription
Method of assay

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line B11 produced by DISSPA, University of Bari, Italy

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 - 1000

Intended Use

in vitro detection of apple mosaic virus (ApMV) in young leaf of pome and stone fruit tree

Specificity

Imed A., Boscia D., Boari A., Saldarelli P., Digiaro M. and Savino V., 1997. A comparison of apple mosaic virus isolates from prunus trees and production of specific monoclonal antibodies. EPPO Bulletin 27, 149

Supplied controls

1 Positive - 1 Negative freeze-dried peach leaf tissue. The absence and the presence of ApMV were ascertained by indexing on peach indicators, and ELISA

Supplied reagents

capture 200 µl/well - detection 200 µl/well - conjugate anti mouse 200 µl/well

Shelf life

9-12 months when stored at 4°C

Sensitivityn.p.

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