ApMV ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line B11 produced by DISSPA, University of Bari, Italy

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of apple mosaic virus (ApMV) in young leaf of pome and stone fruit tree

Imed A., Boscia D., Boari A., Saldarelli P., Digiaro M. and Savino V., 1997. A comparison of apple mosaic virus isolates from prunus trees and production of specific monoclonal antibodies. EPPO Bulletin 27, 149

1 Positive - 1 Negative freeze-dried peach leaf tissue. The absence and the presence of ApMV were ascertained by indexing on peach indicators, and ELISA

capture 200 µl/well - detection 200 µl/well - conjugate anti mouse 200 µl/well

9-12 months when stored at 4°C