PNRSV ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by polyclonal antibodies

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of prunus necrotic ringspot virus (PNRsV) in leaf of pome or stone fruit tree

Cochran,L.C. and Hutchins,L.M. (1941). Phytopathology 31B 860

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PNRsV were ascertained by indexing of peach indicators and ELISA

capture 200 µl/well - detection 200 µl/well - conjugate protein A 200 µl/well

9-12 months when stored at 4°C