PBNSPaV ELISA

ELISA Double Antibody Sandwich (DAS) by polyclonal antibodies

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of plum bark necrosis stem pitting-associated virus (PBNSPaV) in in young leaf of stone fruit tree

Boscia D., O. Potere, M.A. Castellano and V. Savino, 2006. A new antiserum for the detection of plum necrosis stem pitting –associated virus by mean of ELISA.

1 Positive - 1 Negative freeze-dried peach leaf tissue. The absence and the presence of PBNSPaV were ascertained by indexing on peach indicators, PCR and ELISA.

capture 200 µl/well - Conjugate 200 µl/well

9-12 months when stored at 4°C