PPV cherry ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AC produced by DISSPA, University of Bari, Italy

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of plum pox virus type cherry (PPV C) in leaf of stone fruit tree and Nicotiana

Myrta A., Potere O., Crescenzi A., Nuzzaci M. and Boscia D.. 2000. Properties of two monoclonal antibodies specific to the cherry strain of plum pox virus. Journal of Plant Pathology 82 (2), 95-101

1 Positive: freeze dried Nicotiana benthamiana leaf tissue tested for PPV C

1 Negative: freeze-dried peach leaf 

The presence (positive control) and the absence (negative control) of the virus was ascertained by indexing, ELISA and PCR

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C