PPV cherry ELISA

300.00

plum pox virus cherry

capture polyclonal
detection monoclonal line AC
conjugate polyclonal anti mouse
positive and negative controls

  • K-14B
  • PPV cherry ELISA KIT 500 T
  • € 300,00
  • K-14A
  • PPV cherry ELISA KIT 1000 T
  • € 540,00

SKU K-14 Category

Data Sheet

TitleDescription
Method of assay

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AC produced by DISSPA, University of Bari, Italy

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 - 1000

Intended Use

in vitro detection of plum pox virus type cherry (PPV C) in leaf of stone fruit tree and Nicotiana

Specificity

Myrta A., Potere O., Crescenzi A., Nuzzaci M. and Boscia D.. 2000. Properties of two monoclonal antibodies specific to the cherry strain of plum pox virus. Journal of Plant Pathology 82 (2), 95-101

Supplied controls

1 Positive: freeze dried Nicotiana benthamiana leaf tissue tested for PPV C

1 Negative: freeze-dried peach leaf 

The presence (positive control) and the absence (negative control) of the virus was ascertained by indexing, ELISA and PCR

Supplied Reagents

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life

9-12 months when stored at 4°C

Sensitivityn.p.

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