PPV Dideron ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line 4DG5 supplied by Instituto Valenciano de Investigaciones Agrarias (IVIA), Spagna

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of plum pox virus type Dideron (PPV D) in leaf of stone fruit tree

Cambra M., Asensio M., Gorris M.T., Perez E., Camarasa E., Garcia J.A., Moya J.J., Lopez-Abella D., Vela C., and Sanz A., 1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. EPPO Bulletin 24, 569-577

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PPV –D were ascertained by indexing on peeach indicator, ELISA and PCR

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C