PPV El Amar ELISA

300.00

plum pox virus El Amar

capture polyclonal
detection monoclonal line EA24
conjugate polyclonal anti mouse
positive and negative controls

  • K-13B
  • PPV El Amar ELISA KIT 500 T
  • € 300,00
  • K-13A
  • PPV El Amar ELISA KIT 1000 T
  • € 540,00

SKU K-13 Category

Data Sheet

TitleDescription
Method of assay

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line EA24 produced by DIBCA, University of Bari, Italy

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 - 1000

Intended Use

in vitro detection of plum pox virus type El Amar (PPV EA) in leaf of stone fruit tree

Specificity

Myrta A., Potere O., Boscia D., Candresse T., Cambra M., Savino V. 1998. Production of a monoclonal antibody specific to the El Amar strain of plum pox virus. Acta Virologica 42, 248-250

Supplied controls

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PPV –EA were ascertained by indexing on peach indicator, ELISA and PCR

Supplied Reagents

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life

9-12 months when stored at 4°C

Sensitivityn.p.

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