PPV El Amar ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line EA24 produced by DIBCA, University of Bari, Italy

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of plum pox virus type El Amar (PPV EA) in leaf of stone fruit tree

Myrta A., Potere O., Boscia D., Candresse T., Cambra M., Savino V. 1998. Production of a monoclonal antibody specific to the El Amar strain of plum pox virus. Acta Virologica 42, 248-250

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PPV –EA were ascertained by indexing on peach indicator, ELISA and PCR

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C