PPV Marcus ELISA

300.00

plum pox virus Marcus

capture polyclonal
detection monoclonal line AL
conjugate polyclonal anti mouse
positive and negative controls

  • K-11B
  • PPV Marcus ELISA 500 T
  • € 300,00
  • K-11A
  • PPV Marcus ELISA 1000 T
  • € 540,00

SKU k-11 Category

Data Sheet

TitleDescription
Method of assay

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AL produced by DISSPA, University of Bari, Italy

Diagnostic signal

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

Test number

500 - 1000

Intended Use

in vitro detection of plum pox virus type Marcus (PPV M) in leaf of stone fruit tree

Specificity

Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M.T., Myrta A., Di Terlizzi B. and Savino V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.European Journal of Plant Pathology 103, 447-480

Supplied controls

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PPV–M were ascertained by indexing on peach indicator, ELISA and PCR

Supplied Reagents

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

Shelf life

9-12 months when stored at 4°C

Sensitivityn.p.

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