PPV Marcus ELISA

ELISA Double Antibody Sandwich Indirect (DASI) by monoclonal antibody line AL produced by DISSPA, University of Bari, Italy

colorimetric by the action of alkaline phosphatase (AP) on p-nitrophenyl phosphate

500 - 1000

in vitro detection of plum pox virus type Marcus (PPV M) in leaf of stone fruit tree

Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M.T., Myrta A., Di Terlizzi B. and Savino V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus.European Journal of Plant Pathology 103, 447-480

1 Positive - 1 Negative freeze-dried peach leaf. The presence and the absence of PPV–M were ascertained by indexing on peach indicator, ELISA and PCR

capture 200 µl/well - detection 200 µl/well - conjugate 200 µl/well

9-12 months when stored at 4°C